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Acetic acid. a tool to assess anaerobic processes

Acetic acid is one of the central intermediates in anaerobic digestion proceses, and its accumulation mostly indicates a dysfunction of this process. Although acetic acid gives an immediate signal of the physiological status of the anaerobic treatment process, routinely biogas production and pH are usually used as indicators of anaerobic processes because they are easier to measure than acetic acid.

A disadvantage of pH measurement is its dependance on the available buffer-capacity of the sludge. The present paper describes an enzymatic method to measure acetic acid for monitoring the process. The method is based on enzyme-linked test systems. The physiological status of an anaerobic lab scale reactor was monitored using acetic acid, pH and biogas measurements. The system was stressed step by step by “organic overload” with saccharide. Additionally the total cell counts and the cell counts of methanogens and sulfate reducing bateria were monitored. The detection of the bacteria was carried out by Fluorescence In Situ Hybridization (FISH). This test is highly specific and detects bacteria in situ. The aim of the experiments was to seek correlation between acetic acid and process parameters like biogas, organic load and pH.

Copyright:	© European Compost Network ECN e.V.
Quelle:	ORBIT 2003 (Mai 2003)
Seiten:	8
Preis:	€ 0,00
Autor:	PD Dr. rer. nat. Dr.-Ing. habil. Martin Denecke Michalke Klaus
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