Biological production of hydrogen by fermentation in batch and discontinuous tests

Laboratory scale thermophilic fermentation tests were carried out in three different test systems (500 ml Sensomat system, 6 l anaerobic test system (ATS) and a 30 l continuously stirred tank reactor CSTR). Hydrogen was produced from glucose and agricultural products as substrates in batch and discontinuous tests at 60°C. The inoculum consisted of heat-treated digested sewage sludge containing a natural mixed culture. The best specific hydrogen production of 280 Nml H2/g VSS (conversion 112 %) was achieved with glucose at a pH 5.5 in batch operation in the CSTR. Corn starch produced 211 Nml H2/g VSS (conversion 75 %) and potato starch 123 Nml H2/g VSS (conversion 45 %). The agricultural products sugar beet (192 Nml H2/g VSS, 70 %) and fodder beet (185 Nml H2/g VSS, 65 %) also showed good potential for biological hydrogen production. In addition, the fermentation of potato, turnip, corn and bio-waste potato peel resulted in promising hydrogen production rates with conversion yields of 60 %, 50 %, 49 % and 30 %, respectively. In the discontinuous test system, hydrogen production rates of between 0.4 Nl/(lR·d) and 1.3 Nl/(lR·d) were produced, corresponding to yields of between 83 to 445 Nml H2/g VSS.

 

 

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